UJI TOKSISITAS EKSTRAK ETANOL BATANG KAYU WOLE WOE TERHADAP LARVA Artemia salina Leach MENGGUNAKAN METODE Brine Shrimp Lethality Test (BSLT)

The Wole Woe plant is one of the plants that empirically used as traditional medicine by Weda community in Central Halmahera Regency. stem part to treat various diseases. This study aims determine toxicity logs using Brine Shrimp Lethality Test (BSLT) method. research was started extracting ethanol 70% solvent with reflux method and then carried out phytochemical screening which showed contained alkaloids, flavonoids, saponins, triterpenoids, tannins compounds. Toxicity testing wood extract against Artemia salina Leach larvae divided into 5 test groups, namely 4 treatment groups (concentrations 250 ppm, 500 750 1000 ppm) 1 control or comparison group (sea water). Each concentration made 3 vials containing 10 Leach. Observations were looking at mortality after 24 hours treatment. results can be seen through probit analysis calculating LC 50 value. 453.942 ± 59.564 g/mL. indicated ethanolic had a toxic effect on because value was< ppm